抗体发现

A scientist wearing a face mask, safety goggles, and blue gloves is holding a conical flask with an orange cap. The flask contains a pink liquid and some solid particles. In the blurred background of the laboratory, bioanalytical development tools hint at advanced research in absolute bioavailability.

康龙化成抗体发现平台，可为客户提供包括新型和高效单克隆抗体的发现、抗体工程以及抗体表征等服务。我们已成功运用噬菌体展示和杂交瘤两大技术平台，助力新型抗体药物和试剂的研发。

在鼠抗体人源化方面，我们团队拥有丰富的实操经验。该过程旨在保留亲本抗体的结合亲和力和特异性，同时消除异源抗体在患者体内可能引发的免疫原性。此外，我们建立了多种生物物理、生化及细胞检测分析方法，为抗体理化性质和生物活性的表征评估奠定了坚实基础。

研发能力

抗体发现
蛋白质科学
生物大分子药代研究
大分子生物分析
A gloved hand holding a laboratory pipette with multiple channels dispenses pink liquid into the wells of a microplate. The environment appears to be a lab setting, evident from the scientific equipment and precise handling, indicative of bioanalytical development in preclinical ADME studies.

噬菌体展示技术

  • 人源天然库
  • 抗原免疫库
  • 抗体片段库构建
  • 噬菌体文库构建
  • 基于亲和力的淘选与筛选
  • 候选药物确认
  • IgG恒定区转换
  • 定制化鼠、骆驼、羊驼等免疫文库构建
Gloved hands hold a multi-well plate under a microscope for analysis, part of an antibody discovery process in a laboratory setting.

杂交瘤技术

  • 专有免疫方案增强免疫反应
  • 多种免疫方式适应各种免疫原(核酸、短肽、蛋白、细胞或者组合形式)
  • 高效融合方法制备杂交瘤细胞
  • 杂交瘤细胞筛选
  • 阳性克隆基因测序
A scientist in a lab coat, mask, and gloves operates equipment in a sterile laboratory environment. He is handling a sample with a pipette, contributing to bioanalytical development. Another scientist in a lab coat works at a bench in the background. Both focus on their research tasks.

抗体工程

  • 抗体人源化方法：与人源抗体CDR或SDR区链交换，或者人源IgG库筛选
  • 抗体亲和力成熟策略：随机突变、定点突变和其它突变策略相结合
  • 抗体人源化设计：人源抗体数据库、结构模型、以及多种计算机辅助技术
A scientist wearing a lab coat, safety goggles, surgical mask, and blue gloves operates a SEEX IU 500 Plus machine in a laboratory setting. The scientist is intently focused on preclinical ADME studies as they work with the equipment.

抗体表征

  • 亲和力测定（表面等离子共振SPR）
    • 蛋白-蛋白,、蛋白-多肽、蛋白-小分子等多种结合方式的亲和力测定
    • 抗体或Fc融合蛋白与Fc受体结合的亲和力测定
    • 解离速率排序
  • 基于FACS–FACS细胞表面蛋白亲和力测定
  • 生化检测分析
  • 抗原结合检测
    • 蛋白层面：SPR、ELISA
    • 细胞层面：FACS
    • 二价抗体结合检测（蛋白-蛋白,、细胞-蛋白、细胞-细胞）
A person wearing a blue glove operates a laboratory machine with "forteBIO" and "octet" branding, crucial for bioanalytical development. The machine contains black rectangular trays with a grid of wells. A green tray with tubes is also visible. A warning sign advises to keep hands clear of moving parts.

抗体表征

  • 竞争性试验
  • 配体和受体的结合阻断分析（ELISA/FACS)
  • 抗原表位结合位点分析
  • 磷酸化分析
  • HTRF/AlphaLISA
    • 细胞内细胞因子分析（FACS）
  • 细胞水平相关检测
    • 细胞增殖（CFSE/BrdU/CellTiter-Glo®）
    • 细胞筛选（survival/proliferation, 2D和3D culture）
    • 细胞周期检测
    • 细胞凋亡检测
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